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Mark Howarth
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Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
B Zakeri, JO Fierer, E Celik, EC Chittock, U Schwarz-Linek, VT Moy, ...
Proceedings of the National Academy of Sciences 109 (12), E690-E697, 2012
14502012
Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase
I Chen, M Howarth, W Lin, AY Ting
Nature methods 2 (2), 99-104, 2005
8012005
Compact biocompatible quantum dots functionalized for cellular imaging
W Liu, M Howarth, AB Greytak, Y Zheng, DG Nocera, AY Ting, ...
Journal of the American Chemical Society 130 (4), 1274-1284, 2008
7432008
Targeting quantum dots to surface proteins in living cells with biotin ligase
M Howarth, K Takao, Y Hayashi, AY Ting
Proceedings of the National Academy of Sciences 102 (21), 7583-7588, 2005
7282005
Monovalent, reduced-size quantum dots for imaging receptors on living cells
M Howarth, W Liu, S Puthenveetil, Y Zheng, LF Marshall, MM Schmidt, ...
Nature methods 5 (5), 397-399, 2008
5232008
A monovalent streptavidin with a single femtomolar biotin binding site
M Howarth, DJF Chinnapen, K Gerrow, PC Dorrestein, MR Grandy, ...
Nature methods 3 (4), 267-273, 2006
4462006
Site-specific biotinylation of purified proteins using BirA
M Fairhead, M Howarth
Site-Specific Protein Labeling: Methods and Protocols, 171-184, 2015
4062015
Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization
KD Brune, DB Leneghan, IJ Brian, AS Ishizuka, MF Bachmann, SJ Draper, ...
Scientific reports 6 (1), 19234, 2016
3882016
Assembly and antigen-presenting function of MHC class I molecules in cells lacking the ER chaperone calreticulin
B Gao, R Adhikari, M Howarth, K Nakamura, MC Gold, AB Hill, R Knee, ...
Immunity 16 (1), 99-109, 2002
3442002
Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice
AA Cohen, PNP Gnanapragasam, YE Lee, PR Hoffman, S Ou, ...
Science 371 (6530), 735-741, 2021
3212021
Programmable polyproteams built using twin peptide superglues
G Veggiani, T Nakamura, MD Brenner, RV Gayet, J Yan, CV Robinson, ...
Proceedings of the National Academy of Sciences 113 (5), 1202-1207, 2016
3212016
Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher
SC Reddington, M Howarth
Current opinion in chemical biology 29, 94-99, 2015
3162015
Structural analysis and optimization of the covalent association between SpyCatcher and a peptide Tag
L Li, JO Fierer, TA Rapoport, M Howarth
Journal of molecular biology 426 (2), 309-317, 2014
3042014
Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin
M Howarth, AY Ting
Nature protocols 3 (3), 534-545, 2008
2782008
Type 1 insulin-like growth factor receptor translocates to the nucleus of human tumor cells
T Aleksic, MM Chitnis, OV Perestenko, S Gao, PH Thomas, GD Turner, ...
Cancer research 70 (16), 6412-6419, 2010
2422010
Tapasin enhances MHC class I peptide presentation according to peptide half-life
M Howarth, A Williams, AB Tolstrup, T Elliott
Proceedings of the National Academy of Sciences 101 (32), 11737-11742, 2004
2352004
A streptavidin variant with slower biotin dissociation and increased mechanostability
CE Chivers, E Crozat, C Chu, VT Moy, DJ Sherratt, M Howarth
Nature methods 7 (5), 391-393, 2010
2282010
A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses
TK Tan, P Rijal, R Rahikainen, AH Keeble, L Schimanski, S Hussain, ...
Nature communications 12 (1), 542, 2021
2272021
How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer
CE Chivers, AL Koner, ED Lowe, M Howarth
Biochemical Journal 435 (1), 55-63, 2011
2112011
SpyLigase peptide–peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture
JO Fierer, G Veggiani, M Howarth
Proceedings of the National Academy of Sciences 111 (13), E1176-E1181, 2014
2022014
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