Authors
Salvador J Díaz-Cano, Stephen P Brady
Publication date
1997/12/1
Journal
Diagnostic Molecular Pathology
Volume
6
Issue
6
Pages
342-346
Publisher
LWW
Description
Abstract Several DNA extraction methods have been used for formalinfixed, paraffin-
embedded tissues, with variable results being reported regarding the suitability of DNA
obtained from such sources to serve as template in polymerase chain reaction (PCR)-based
genetic analyses. We present a method routinely used for archival material in our laboratory
that reliably yields DNA of sufficient quality for PCR studies. This method is based on
extended proteinase K digestion (250 [mu] g/ml in an EDTA-free calcium-containing buffer ...
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