Alexander W Bell, Eric W Deutsch, Catherine E Au, Robert E Kearney, Ron Beavis, Salvatore Sechi, Tommy Nilsson, John JM Bergeron, Thomas A Beardslee, Thomas Chappell, Gavin Meredith, Peter Sheffield, Phillip Gray, Mahbod Hajivandi, Marshall Pope, Paul Predki, Majlinda Kullolli, Marina Hincapie, William S Hancock, Wei Jia, Lina Song, Lei Li, Junying Wei, Bing Yang, Jinglan Wang, Wantao Ying, Yangjun Zhang, Yun Cai, Xiaohong Qian, Fuchu He, Helmut E Meyer, Christian Stephan, Martin Eisenacher, Katrin Marcus, Elmar Langenfeld, Caroline May, Steven A Carr, Rushdy Ahmad, Wenhong Zhu, Jeffrey W Smith, Samir M Hanash, Jason J Struthers, Hong Wang, Qing Zhang, Yanming An, Radoslav Goldman, Elisabet Carlsohn, Sjoerd Van Der Post, Kenneth E Hung, David A Sarracino, Kenneth Parker, Bryan Krastins, Raju Kucherlapati, Sylvie Bourassa, Guy G Poirier, Eugene Kapp, Heather Patsiouras, Robert Moritz, Richard Simpson, Benoit Houle, Sylvie LaBoissiere, Pavel Metalnikov, Vivian Nguyen, Tony Pawson, Catherine CL Wong, Daniel Cociorva, John R Yates III, Michael J Ellison, Ana Lopez-Campistrous, Paul Semchuk, Yueju Wang, Peipei Ping, Giuliano Elia, Michael J Dunn, Kieran Wynne, Angela K Walker, John R Strahler, Philip C Andrews, Brian L Hood, William L Bigbee, Thomas P Conrads, Derek Smith, Christoph H Borchers, Gilles A Lajoie, Sean C Bendall, Kaye D Speicher, David W Speicher, Masanori Fujimoto, Kazuyuki Nakamura, Young-Ki Paik, Sang Yun Cho, Min-Seok Kwon, Hyoung-Joo Lee, Seul-Ki Jeong, An Sung Chung, Christine A Miller, Rudolf Grimm, Katy Williams, Craig Dorschel, Jayson A Falkner, Lennart Martens, Juan Antonio Vizcaíno

2009/6/1

Nature methods

6

6

423-430

Nature Publishing Group

Abstract We performed a test sample study to try to identify errors leading to irreproducibility,
including incompleteness of peptide sampling, in liquid chromatography–mass spectrometry–
based proteomics. We distributed an equimolar test sample, comprising 20 highly purified
recombinant human proteins, to 27 laboratories. Each protein contained one or more unique
tryptic peptides of 1,250 Da to test for ion selection and sampling in the mass spectrometer.
Of the 27 labs, members of only 7 labs initially reported all 20 proteins correctly, and ...