José J. Virgen Ortíz
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Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions
RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos, Á Berenguer-Murcia, ...
Biotechnology advances 37 (5), 746-770, 2019
Effect of protein load on stability of immobilized enzymes
L Fernandez-Lopez, SG Pedrero, N Lopez-Carrobles, BC Gorines, ...
Enzyme and microbial technology 98, 18-25, 2017
Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts
JJ Virgen-Ortíz, JCS Dos Santos, Á Berenguer-Murcia, O Barbosa, ...
Journal of Materials Chemistry B 5 (36), 7461-7490, 2017
Effect of immobilization rate and enzyme crowding on enzyme stability under different conditions. The case of lipase from Thermomyces lanuginosus immobilized on octyl agarose beads
H Zaak, EH Siar, JF Kornecki, L Fernandez-Lopez, SG Pedrero, ...
Process Biochemistry 56, 117-123, 2017
Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil: Critical role of the immobilization support
VG Tacias-Pascacio, JJ Virgen-Ortíz, M Jiménez-Pérez, M Yates, ...
Fuel 200, 1-10, 2017
Relevance of substrates and products on the desorption of lipases physically adsorbed on hydrophobic supports
JJ Virgen-Ortíz, VG Tacias-Pascacio, DB Hirata, B Torrestiana-Sanchez, ...
Enzyme and microbial technology 96, 30-35, 2017
Evaluation of different immobilized lipases in transesterification reactions using tributyrin: Advantages of the heterofunctional octyl agarose beads
DB Hirata, TL Albuquerque, N Rueda, JJ Virgen-Ortíz, ...
Journal of Molecular Catalysis B: Enzymatic 133, 117-123, 2016
Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a β-galactosidase
S Peirce, JJ Virgen-Ortíz, VG Tacias-Pascacio, N Rueda, ...
RSC advances 6 (66), 61707-61715, 2016
Evaluation of different commercial hydrophobic supports for the immobilization of lipases: tuning their stability, activity and specificity
VG Tacias-Pascacio, S Peirce, B Torrestiana-Sanchez, M Yates, ...
RSC advances 6 (102), 100281-100294, 2016
Comparison of acid, basic and enzymatic catalysis on the production of biodiesel after RSM optimization
VG Tacias-Pascacio, B Torrestiana-Sánchez, L Dal Magro, JJ Virgen-Ortíz, ...
Renewable Energy 135, 1-9, 2019
Physical crosslinking of lipase from Rhizomucor miehei immobilized on octyl agarose via coating with ionic polymers: Avoiding enzyme release from the support
L Fernandez-Lopez, SG Pedrero, N Lopez-Carrobles, JJ Virgen-Ortíz, ...
Process Biochemistry 54, 81-88, 2017
Reuse of anion exchangers as supports for enzyme immobilization: Reinforcement of the enzyme-support multiinteraction after enzyme inactivation
JJ Virgen-Ortíz, S Peirce, VG Tacias-Pascacio, V Cortes-Corberan, ...
Process Biochemistry 51 (10), 1391-1396, 2016
Method to concentrate protein solutions based on dialysis–freezing–centrifugation: Enzyme applications
JJ Virgen-Ortíz, V Ibarra-Junquera, JA Osuna-Castro, ...
Analytical biochemistry 426 (1), 4-12, 2012
Stabilization of Candida antarctica lipase B (CALB) immobilized on octyl agarose by treatment with polyethyleneimine (PEI)
S Peirce, VG Tacias-Pascacio, ME Russo, A Marzocchella, JJ Virgen-Ortíz, ...
Molecules 21 (6), 751, 2016
Desorption of lipases immobilized on octyl-agarose beads and coated with ionic polymers after thermal inactivation. Stronger adsorption of polymers/unfolded protein composites
JJ Virgen-Ortíz, SG Pedrero, L Fernandez-Lopez, N Lopez-Carrobles, ...
Molecules 22 (1), 91, 2017
Optimization of the coating of octyl-CALB with ionic polymers to improve stability and decrease enzyme leakage
L Fernandez-Lopez, JJ Virgen-OrtÍz, SG Pedrero, N Lopez-Carrobles, ...
Biocatalysis and Biotransformation 36 (1), 47-56, 2018
Kinetics and thermodynamic of the purified dextranase from Chaetomium erraticum
JJ Virgen-Ortíz, V Ibarra-Junquera, P Escalante-Minakata, JJ Ornelas-Paz, ...
Journal of Molecular Catalysis B: Enzymatic 122, 80-86, 2015
Lecitase ultra: A phospholipase with great potential in biocatalysis
JJ Virgen-Ortíz, JCS dos Santos, C Ortiz, Á Berenguer-Murcia, O Barbosa, ...
Molecular Catalysis 473, 110405, 2019
Improving sodium dodecyl sulfate polyacrylamide gel electrophoresis detection of low-abundance protein samples by rapid freeze centrifugation
JJ Virgen-Ortíz, V Ibarra-Junquera, P Escalante-Minakata, ...
Analytical biochemistry 443 (2), 249-251, 2013
Identification and functional characterization of a fructooligosaccharides-forming enzyme from Aspergillus aculeatus
JJ Virgen-Ortíz, V Ibarra-Junquera, P Escalante-Minakata, ...
Applied biochemistry and biotechnology 179 (3), 497-513, 2016
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