Glutathione S-transferases (GSTs) from the phi (GSTF) and tau (GSTU) classes are unique
to plants and play important roles in stress tolerance and secondary metabolism as well as
catalyzing the detoxification of herbicides in crops and weeds. We have cloned and …

Abstract The LH/CG receptor (LH/CG-R) belongs to the family of glycoprotein hormone G
protein-coupled receptors. The extracellular domain of LH/CG-R is associated with high
ligand-binding affinity and contains leucine-rich repeats (LRRs). With the goal of identifying …

The cis-trans isomerisation of maleylacetoacetate to fumarylacetoacetate is the penultimate
step in the tyrosine/phenylalanine catabolic pathway and has recently been shown to be
catalysed by glutathione S-transferase enzymes belonging to the zeta class. Given this …

Plant Tau class glutathione transferases (GSTUs) detoxify diphenylether herbicides such as
fluorodifen, determining their selectivity in crops and weeds. Using reconstructive PCR, a
series of mutant GSTUs were generated from in vitro recombination and mutagenesis of the …

Shikimate dehydrogenase catalyzes the fourth step of the shikimate pathway, the essential
route for the biosynthesis of aromatic compounds in plants and microorganisms. Absent in
metazoans, this pathway is an attractive target for nontoxic herbicides and drugs …

The three-dimensional structure of shikimate kinase from Erwinia chrysanthemi has been
determined by multiple isomorphous replacement. Two models are presented: a high
resolution 1.9 Å model and a 2.6 Å model which contains bound Mg-ADP. The enzyme is an …

Low-frequency collective vibrational modes in proteins have been proposed as being
responsible for efficiently directing biochemical reactions and biological energy transport.
However, evidence of the existence of delocalized vibrational modes is scarce and proof of …

The structure of the type II DHQase from Streptomyces coelicolor has been solved and
refined to high resolution in complexes with a number of ligands, including
dehydroshikimate and a rationally designed transition state analogue, 2, 3-anhydro-quinic …

Shikimate kinase, despite low sequence identity, has been shown to be structurally a
member of the nucleoside monophosphate (NMP) kinase family, which includes adenylate
kinase. In this paper we have explored the roles of residues in the P‐loop of shikimate …

The structure of the rat liver aflatoxin dialdehyde reductase (AKR7A1) has been solved to
1.38-Å resolution. Although it shares a similar α/β-barrel structure with other members of the
aldo-keto reductase superfamily, AKR7A1 is the first dimeric member to be crystallized. The …