Many ATP-and GTP-binding proteins have a phosphate-binding loop (P-loop), the primary
structure of which typically consists of a glycine-rich sequence followed by a conserved
lysine and a serine or threonine. The three-dimensional structures of several ATP-and GTP …

Rap1 is a small, Ras-like GTPase that was first identified as a protein that could suppress
the oncogenic transformation of cells by Ras 1. Rap1 is activated by several extracellular
stimuli 2, 3, 4, 5, 6, 7 and may be involved in cellular processes such as cell proliferation 8 …

Guanine nucleotide–binding proteins regulate a variety of processes, including sensual
perception, protein synthesis, various transport processes, and cell growth and
differentiation. They act as molecular switches and timers that cycle between inactive …

The three-dimensional structure of the complex between human H-Ras bound to guanosine
diphosphate and the guanosine triphosphatase (GTPase)–activating domain of the human
GTPase-activating protein p120 GAP (GAP-334) in the presence of aluminum fluoride was …

Guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs)
regulate the activity of small guanine nucleotide-binding (G) proteins to control cellular
functions. In general, GEFs turn on signaling by catalyzing the exchange from G-protein …

The crystal structure of the H‐ras oncogene protein p21 complexed to the slowly hydrolysing
GTP analogue GppNp has been determined at 1.35 A resolution. 211 water molecules have
been built into the electron density. The structure has been refined to a final R‐factor of …

The crystal structure of the guanine-nucleotide-binding domain of p21 (amino acids 1–166)
complexed to the guanosine triphosphate analogue guanosine-5′-(β, γ-imido) triphosphate
(GppNp) has been determined at a resolution of 2.6 Å. The topological order of secondary …

We show that the specific subcellular distribution of H-and Nras guanosine triphosphate–
binding proteins is generated by a constitutive de/reacylation cycle that operates on
palmitoylated proteins, driving their rapid exchange between the plasma membrane (PM) …

The X-ray crystal structure of the complex between the Ras-related protein RaplA in the GTP-
analogue (GppNHp) form and the Ras-binding domain (RBD) of the Ras effector molecule c-
Raf1, a Ser/Thr-specific protein kinase, has been solved to a resolution of 2.2 Å. It shows that …

The early discovery of ras genes was due to their highly oncogenic potential when
transduced in retroviruses, such as the Harvey and Kirsten murine sarcoma viruses
containing viral forms of H-ras and K-ras, respectively. Variants of these two genes and of a …