THE p53 tumour-suppressor protein controls the expression of a gene encoding the p21
cyclin-dependent protein kinase (CDK) regulator 1–6. Levels of p21 protein are increased in
senescent cells and p21 overexpression blocks the growth of tumour cells 1, 3, 7. In normal …

A multiprotein complex that specifically recognizes cellular origins of DNA replication has
been identified and purified from the yeast Saccharomyces cerevisiae. We observe a strong
correlation between origin function and origin recognition by this activity. Interestingly …

The mechanism of replication of the simian virus 40 (SV40) genome closely resembles that
of cellular chromosomes, thereby providing an excellent model system for examining the
enzymatic requirements for DNA replication 1. Only one viral gene product, the large tumour …

Replication of the two template strands at eukaryotic cell DNA replication forks is a highly
coordinated process that ensures accurate and efficient genome duplication. Biochemical
studies, principally of plasmid DNAs containing the Simian Virus 40 origin of DNA …

Evidence obtained from studies with yeast and Xenopusindicate that the initiation of DNA
replication is a multistep process. The origin recognition complex (ORC), Cdc6p, and
minichromosome maintenance (MCM) proteins are required for establishing prereplication …

IN mammalian cells, DNA damage increases the levels of the nuclear tumour-suppressor
p53, resulting in elevated synthesis of p21, an inhibitor of cyclin-dependent kinases (CDK) 1
6. p21 may also directly block DNA replication by inhibiting the proliferating-cell nuclear …

Although it has been demonstrated that discrete origins of DNA replication exist in
eukaryotic cellular chromosomes, the detailed organization of a eukaryotic cellular origin
remains to be determined. Linker substitution mutations were constructed across the entire …

Abstract Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically
determined chromosomal states in vivo and promotes assembly of chromatin during DNA
replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not …

Chromatin assembly factor 1 (CAF-1) assembles nucleosomes in a replication-dependent
manner. The small subunit of CAF-1 (p48) is a member of a highly conserved subfamily of
WD-repeat proteins. There are at least two members of this subfamily in both human (p46 …

The purification and characterization of a replication-dependent chromatin assembly factor
(CAF-I) from the nuclei of human cells is described. CAF-I is a multisubunit protein that,
when added to a crude cytosol replication extract, promotes chromatin assembly on …