Millisecond-timescale, genetically targeted optical control of neural activity
Temporally precise, noninvasive control of activity in well-defined neuronal populations is a
long-sought goal of systems neuroscience. We adapted for this purpose the naturally
occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel …
long-sought goal of systems neuroscience. We adapted for this purpose the naturally
occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel …
High-performance genetically targetable optical neural silencing by light-driven proton pumps
…, GM Belfort, Y Lin, PE Monahan, ES Boyden - Nature, 2010 - nature.com
The ability to silence the activity of genetically specified neurons in a temporally precise
fashion would provide the opportunity to investigate the causal role of specific cell classes in
neural computations, behaviours and pathologies. Here we show that members of the class …
fashion would provide the opportunity to investigate the causal role of specific cell classes in
neural computations, behaviours and pathologies. Here we show that members of the class …
Channelrhodopsin-2 and optical control of excitable cells
Electrically excitable cells are important in the normal functioning and in the
pathophysiology of many biological processes. These cells are typically embedded in
dense, heterogeneous tissues, rendering them difficult to target selectively with conventional …
pathophysiology of many biological processes. These cells are typically embedded in
dense, heterogeneous tissues, rendering them difficult to target selectively with conventional …
Independent optical excitation of distinct neural populations
Optogenetic tools enable examination of how specific cell types contribute to brain circuit
functions. A long-standing question is whether it is possible to independently activate two
distinct neural populations in mammalian brain tissue. Such a capability would enable the …
functions. A long-standing question is whether it is possible to independently activate two
distinct neural populations in mammalian brain tissue. Such a capability would enable the …
A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing
Cell type–specific expression of optogenetic molecules allows temporally precise
manipulation of targeted neuronal activity. Here we present a toolbox of four knock-in mouse
lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2 …
manipulation of targeted neuronal activity. Here we present a toolbox of four knock-in mouse
lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2 …
Steerable surgical stapler
ES Boyden, RA Hyde, MY Ishikawa… - US Patent …, 2010 - Google Patents
Embodiments include a surgical device and a method. An embodiment of the surgical
instrument includes at least one grasping jaw, the at least one grasping jaw being adapted
to deliver surgical staples by a force generated from a force generator mechanism that is …
instrument includes at least one grasping jaw, the at least one grasping jaw being adapted
to deliver surgical staples by a force generated from a force generator mechanism that is …
[HTML][HTML] Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution
The quest to determine how precise neural activity patterns mediate computation, behavior,
and pathology would be greatly aided by a set of tools for reliably activating and inactivating
genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having …
and pathology would be greatly aided by a set of tools for reliably activating and inactivating
genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having …
Gentle touch surgical stapler
ES Boyden, RA Hyde, MY Ishikawa… - US Patent …, 2010 - Google Patents
Embodiments include a surgical device and a method. An embodiment of the surgical
instrument includes at least one grasping jaw, the at least one grasping jaw being adapted
to deliver surgical staples by a force generated from a force generator mechanism that is …
instrument includes at least one grasping jaw, the at least one grasping jaw being adapted
to deliver surgical staples by a force generated from a force generator mechanism that is …
[HTML][HTML] Millisecond-timescale optical control of neural dynamics in the nonhuman primate brain
…, RT Bronson, AM Graybiel, R Desimone, ES Boyden - Neuron, 2009 - Elsevier
To understand how brain states and behaviors are generated by neural circuits, it would be
useful to be able to perturb precisely the activity of specific cell types and pathways in the
nonhuman primate nervous system. We used lentivirus to target the light-activated cation …
useful to be able to perturb precisely the activity of specific cell types and pathways in the
nonhuman primate nervous system. We used lentivirus to target the light-activated cation …
Maneuverable surgical stapler
ES Boyden, RA Hyde, MY Ishikawa… - US Patent …, 2012 - Google Patents
Embodiments include a surgical device and a method. An embodiment of the surgical
instrument includes at least one grasping jaw, the at least one grasping jaw being adapted
to deliver surgical staples by a force generated from a force generator mechanism that is …
instrument includes at least one grasping jaw, the at least one grasping jaw being adapted
to deliver surgical staples by a force generated from a force generator mechanism that is …
