The KRAS oncogene product is considered a major target in anticancer drug discovery 1, 2,
3. However, direct interference with KRAS signalling has not yet led to clinically useful drugs
3, 4, 5, 6, 7, 8. Correct localization and signalling by farnesylated KRAS is regulated by the …

Abstract Lipidated Rho and Rab GTP-binding proteins are transported between membranes
in complex with solubilizing factors called'guanine nucleotide dissociation inhibitors'(GDIs).
Unloading from GDIs using GDI displacement factors (GDFs) has been proposed but …

Understanding protein structure and function is essential for uncovering the secrets of
biology, but it remains extremely challenging because of the high complexity of protein
networks and their wiring. The daunting task of elucidating these interconnections requires …

In a combined chemical biological and biophysical approach using time-lapse tapping-
mode atomic force microscopy, we studied the partitioning of differently lipidated N-Ras
proteins with various membrane-localization motifs into lipid domains of canonical model raft …

Cellular proteome labeling profile for probes 3 and 4 derived from palmostatin M after 20 min
incubation at various probe concentrations (50, 10, 1 μM). A) Fluorescent gel highlighting three
major bands corresponding to PPT1 (34.193 kDa), APT1 (MW=24.67 kDa), and APT2 (MW=24.73 …

The K-Ras4B GTPase is a major oncoprotein whose sig-naling activity depends on its
correct localization to negatively charged subcellular membranes and nanoclustering in
membrane microdomains. Selective localization and clustering are mediated by the …

Ras is a central regulator of cellular signaling pathways. It is mutated in 20–30% of human
tumors. To perform its function, Ras has to be bound to a membrane by a posttranslationally
attached lipid anchor. Surprisingly, we identified here dimerization of membrane anchored …

Regulation of protein function is often linked to a conformational switch triggered by
chemical or physical signals. To evaluate such conformational changes and to elucidate the
underlying molecular mechanisms of subsequent protein function, experimental …

The synthesis of several analogues of the N-[(1 R, 2 S)-2-hydroxy-1-hydroxymethyl-2-(2-
tridecyl-1-cyclopropenyl) ethyl] octanamide (GT11), the first reported inhibitor of
dihydroceramide desaturase, as well as their effects on this enzyme, are described …

A new linker: In a facile, chemoselective, and potentially general method for protein
modification at the C terminus oxyamino‐modified proteins obtained from protein thioesters
react rapidly with ketones under mild conditions. This strategy was used for fluorescence …