Brown fat generates heat via the mitochondrial uncoupling protein UCP1, defending against
hypothermia and obesity. Recent data suggest that there are two distinct types of brown fat:
classical brown fat derived from a myf-5 cellular lineage and UCP1-positive cells that …

Satellite cells (SC) are essential for skeletal muscle growth and repair. Because sarcopenia
is associated with type II muscle fiber atrophy, we hypothesized that SC content is
specifically reduced in the type II fibers in the elderly. A total of eight elderly (E; 76±1 yr) and …

The objective of the present study was to develop a stain permitting automated quantification
of myocellular lipid depositions in skeletal muscle sections together with immunolocalisation
of other myocellular constituents by fluorescence microscopy. Lipid droplets were detected …

Monoclonal antibody (RCK 105) directed against keratin 7 was obtained after immunization
of BALB/c mice with cytoskeletal preparations from T24 cells and characterized by one-(1D)
and two-dimensional (2D) immunoblotting. In cultured epithelial cells, known from gel …

The characterization of a novel 59-kD cytoskeletal protein is described. It is exclusively
observed in smooth muscle cells by Northern blotting and immunohistochemical analysis
and therefore designated" smoothelin." A human smooth muscle cDNA library was screened …

Objective-Mitochondrial dysfunction and fat accumulation in skeletal muscle (IMCL) has
been linked to development of type 2 diabetes. We examined if exercise training could
restore mitochondrial function and insulin sensitivity in patients with type 2 diabetes (T2D) …

The nuclear receptor Rev-erb-α modulates hepatic lipid and glucose metabolism,
adipogenesis and the inflammatory response in macrophages. We show here that Rev-erb-
α is highly expressed in oxidative skeletal muscle and that its deficiency in muscle leads to …

SPECIFIC AIMS There is increasing evidence that uncoupling protein 3, in contrast to brown
adipose tissue-specific UCP1, is not playing a major role in energy dissipation but rather is
involved in fatty acid metabolism. We and others have recently postulated the hypothesis …

Cold exposure may be a potential therapy for diabetes by increasing brown adipose tissue
(BAT) mass and activity. Here we report that 10 d of cold acclimation (14–15 C) increased
peripheral insulin sensitivity by∼ 43% in eight type 2 diabetes subjects. Basal skeletal …

Recently, a role for uncoupling protein-3 (UCP3) in carbohydrate metabolism and in type 2
diabetes has been suggested. Mice overexpressing UCP3 in skeletal muscle showed
reduced fasting plasma glucose levels, improved glucose tolerance after an oral glucose …