Already, devices that harness electrical impulses are used to treat disease. Pacemakers and
defibrillators save millions of lives each year; deep-brain stimulation dramatically improves
the quality of life for people with Parkinson's disease and depress ion; sacral-nerve …

We describe a method for selecting aggregation-resistant proteins by heat denaturation.
This is illustrated with antibody heavy chain variable domains (dAbs), which are prone to
aggregate 1, 2. The dAbs were displayed multivalently at the infective tip of filamentous …

The current predominant therapeutic paradigm is based on maximizing drug-receptor
occupancy to achieve clinical benefit. This strategy, however, generally requires excessive
drug concentrations to ensure sufficient occupancy, often leading to adverse side effects …

With the rapid rise in technology for the precision detection and modulation of electrical
signalling patterns in the nervous system, a new class of treatments known as bioelectronic
medicines seems within reach1. Specifically, the peripheral nervous system will be at the …

Protein aggregates are usually formed by interactions between unfolded or partially
unfolded species, and often occur when a protein is denatured by, for example, heat or low
pH. In earlier work, we used a Darwinian selection strategy to create human antibody …

We recently described a method for the generation of a large human domain antibody
repertoire involving combinatorial assembly of CDR building blocks from a smaller
repertoire comprising a high frequency of aggregation-resistant antibody domains. Here we …

Human antibody variable heavy (VH) domains tend to aggregate upon denaturation, for
instance, by heat or acid. We have previously demonstrated that domains resisting protein
aggregation can be selected from CDR-only repertoires by phage display. Here we …

Molecular evolution is a powerful means of engineering proteins. It usually requires the
generation of a large recombinant DNA library of variants for cloning into a phage or plasmid
vector, and the transformation of a host organism for expression and screening of the variant …

The vitamin B6-dependent enzyme 7, 8-diaminopelargonic acid (DAPA) synthase catalyzes
the antepenultimate step in the synthesis of biotin, the transfer of the α-amino group of S-
adenosyl-l-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form DAPA. The …

The invention relates to polypeptides that unfold reversibly (eg, unfolds when heated and
refolds when cooled), to repertoires containing polypeptides that unfold reversibly and to
libraries that contain polypeptides that unfold reversibly or nucleic acids that encode …