There is increased demand for nanoparticles with a high fluorescence yield that have the
desired excitation wavelength, surface functionalization, and particle size to act as biological
probes. Here, a simple, rapid, and robust method, Flash NanoPrecipitation (FNP), to …

We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons
and introns on individual RNA transcripts with high efficiency. We used this method to
quantify allelic expression in cell populations and in single cells, and also to distinguish …

We investigate numerically the structure, thermodynamics, and relaxation behavior of a
family of (n, 6) Lennard-Jones-like glass-forming binary mixtures interacting via pair
potentials with variable softness, fixed well depth, and fixed well depth location. These …

During mitosis, RNA polymerase II (Pol II) and many transcription factors dissociate from
chromatin, and transcription ceases globally. Transcription is known to restart in bulk by
telophase, but whether de novo transcription at the mitosis–G1 transition is in any way …

Imprinting is a classic mammalian epigenetic phenomenon that results in expression from a
single parental allele. Imprinting defects can lead to inappropriate expression from the
normally silenced allele, but it remains unclear whether every cell in a mutant organism …

Figure 1 Choosing the proper assay for gene expression involves trade-offs on parameters
such as throughput or positional resolution. Northern blots assay a few genes at a time and
average over bulk samples. RNA-seq provides tremendous throughput, but positional …

The tremendous proliferative capacity of erythroid precursor cells underlies the production of
over a million red blood cells per second in adult humans. During every mitosis the
mammalian nucleus is disassembled and transcriptionally silent. Genome reactivation after …

Single molecule RNA FISH techniques have enabled the quantification of gene expression
at the single cell level, revealing significant variability that has been previously obscured by
techniques measuring population averages. These techniques, however, have been limited …

SM Alam, Kansas State University, USA Alicia Allen, National Renewable Energy Laboratory
(NREL), USA Shingo Ata, Osaka City University, Japan Hamed Babazadehrokni, Colorado Energy
Research Technologies (CERT), USA Amos Brocco, Karlsruher Institut für Technologie …