Novel nanomaterials for bioassay applications represent a rapidly progressing field of
nanotechnology and nanobiotechnology. Here, we present an exploration of single-walled
carbon nanotubes as a platform for investigating surface–protein and protein–protein …

Molecules involved in the antigen receptor-dependent regulation of early T cell activation
genes were investigated with the use of functional sequences of the T cell activation-specific
enhancer of interleukin-2 (IL-2). One of these sequences forms a protein complex, NFAT-1 …

We constructed miniaturized autoantigen arrays to perform large-scale multiplex
characterization of autoantibody responses directed against structurally diverse
autoantigens, using submicroliter quantities of clinical samples. Autoantigen microarrays …

Inhibitory receptors on immune cells are pivotal regulators of immune escape in cancer.
Among these inhibitory receptors, CTLA-4 (targeted clinically by ipilimumab) serves as a
dominant off-switch while other receptors such as PD-1 and LAG-3 seem to serve more …

Nuclear processes such as transcription, DNA replication and recombination are
dynamically regulated by chromatin structure. Eukaryotic transcription is known to be
regulated by chromatin-associated proteins containing conserved protein domains that …

The current sensitivity of standard fluorescence-based protein detection limits the use of
protein arrays in research and clinical diagnosis. Here, we use functionalized,
macromolecular single-walled carbon nanotubes (SWNTs) as multicolor Raman labels for …

Objective Because rheumatoid arthritis (RA) is a heterogeneous autoimmune disease in
terms of disease manifestations, clinical outcomes, and therapeutic responses, we
developed and applied a novel antigen microarray technology to identify distinct serum …

The diversity of autoimmune responses poses a formidable challenge to the development of
antigen-specific tolerizing therapy. We developed'myelin proteome'microarrays to profile the
evolution of autoantibody responses in experimental autoimmune encephalomyelitis (EAE) …

We have developed an acrylic microfluidic device that sequentially couples liquid-phase
isoelectric focusing (IEF) and free solution capillary electrophoresis (CE). Rapid separation
(< 1 min) and preconcentration (73×) of species were achieved in the initial IEF dimension …

Constitutive Notch activation is required for the proliferation of a subgroup of T-cell acute
lymphoblastic leukemia (T-ALL). Downstream pathways that transmit pro-oncogenic signals
are not well characterized. To identify these pathways, protein microarrays were used to …