The long-term challenge of proteomics is enormous: to define the identities, quantities,
structures and functions of complete complements of proteins, and to characterize how these
properties vary in different cellular contexts. One critical step in tackling this goal is the …

We show that the specific subcellular distribution of H-and Nras guanosine triphosphate–
binding proteins is generated by a constitutive de/reacylation cycle that operates on
palmitoylated proteins, driving their rapid exchange between the plasma membrane (PM) …

Fluorescence lifetime imaging microscopy (FLIM) is a technique in which the mean
fluorescence lifetime of a chromophore is measured at each spatially resolvable element of
a microscope image. The nanosecond excited-state lifetime is independent of probe …

The mitogen-activated protein kinase (MAPK) network is a conserved signalling module that
regulates cell fate by transducing a myriad of growth-factor signals 1. The ability of this
network to coordinate and process a variety of inputs from different growth-factor receptors …

Proteins provide the building blocks for multicomponent molecular units, or pathways, from
which higher cellular functions emerge. These units consist of either assemblies of
physically interacting proteins or dispersed biochemical activities connected by rapidly …

When bound by extracellular ligands, receptor tyrosine kinases (RTKs) on the cell surface
transmit critical signals to the cell interior. Although signal termination is less well
understood, protein tyrosine phosphatase–1B (PTP1B) is implicated in the …

Protein kinase C (PKC) has been implicated in integrin‐mediated spreading and migration.
In mammary epithelial cells there is a partial co‐localization between β1 integrin and PKCα.
This reflects complexes between these proteins as demonstrated by fluorescense …

Evidence for a new signaling mechanism consisting of ligand-independent lateral
propagation of receptor activation in the plasma membrane is presented. We visualized the
phosphorylation of green fluorescent protein (GFP)–tagged ErbB1 (ErbB1-GFP) receptors in …

The KRAS oncogene product is considered a major target in anticancer drug discovery 1, 2,
3. However, direct interference with KRAS signalling has not yet led to clinically useful drugs
3, 4, 5, 6, 7, 8. Correct localization and signalling by farnesylated KRAS is regulated by the …

Spatially resolved fluorescence resonance energy transfer (FRET) measured by
fluorescence lifetime imaging microscopy (FLIM), provides a method for tracing the catalytic
activity of fluorescently tagged proteins inside live cell cultures and enables determination of …