The KRAS oncogene product is considered a major target in anticancer drug discovery 1, 2,
3. However, direct interference with KRAS signalling has not yet led to clinically useful drugs
3, 4, 5, 6, 7, 8. Correct localization and signalling by farnesylated KRAS is regulated by the …

We identify a role for the GDI-like solubilizing factor (GSF) PDEδ in modulating signalling
through Ras family G proteins by sustaining their dynamic distribution in cellular
membranes. We show that the GDI-like pocket of PDEδ binds and solubilizes farnesylated …

Abstract Lipidated Rho and Rab GTP-binding proteins are transported between membranes
in complex with solubilizing factors called'guanine nucleotide dissociation inhibitors'(GDIs).
Unloading from GDIs using GDI displacement factors (GDFs) has been proposed but …

The M1T1 strain remains the most frequently isolated strain from group A streptococcal
(GAS) infection cases worldwide. We previously reported that M1T1 differs from the fully
sequenced M1 SF370 strain. To better understand the reason for the persistence and …

Access to the ciliary membrane for trans‐membrane or membrane‐associated proteins is a
regulated process. Previously, we have shown that the closely homologous small G proteins
Arl2 and Arl3 allosterically regulate prenylated cargo release from PDEδ. UNC119/HRG4 is …

Arfs are small G proteins that have a key role in vesicle trafficking and cytoskeletal
remodeling. ArfGAP proteins stimulate Arf intrinsic GTP hydrolysis by a mechanism that is
still unresolved. Using a fusion construct we solved the structure of the ArfGAP ASAP3 in …

MINT‐6602303: ARL2 (uniprotkb: P36404) binds (MI: 0407) to HRG4 (uniprotkb: Q13432)
by pull down (MI: 0096) MINT‐6602333: ARL3 (uniprotkb: P36405) binds (MI: 0407) to CoD
(uniprotkb: Q9BTW9) by pull down (MI: 0096) MINT‐6602347, MINT‐6602369: RP2 …

The prenyl-binding protein PDEδ is crucial for the plasma membrane localization of
prenylated Ras. Recently, we have reported that the small-molecule Deltarasin binds to the
prenyl-binding pocket of PDEδ, and impairs Ras enrichment at the plasma membrane …

Defects in primary cilia result in human diseases known as ciliopathies. The retinitis
pigmentosa GTPase regulator (RPGR), mutated in the most severe form of the eye disease,
is located at the transition zone of the ciliary organelle. The RPGR‐interacting partner PDEδ …

The crystal structure of human liver glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
has been determined. This structure represents the first moderate-resolution (2.5 Å) and
crystallographically refined (Rfree= 22.9%) human GAPDH structure. The liver GAPDH …