Inhibitory receptors on immune cells are pivotal regulators of immune escape in cancer.
Among these inhibitory receptors, CTLA-4 (targeted clinically by ipilimumab) serves as a
dominant off-switch while other receptors such as PD-1 and LAG-3 seem to serve more …

Signaling via the methylation of lysine residues in proteins has been linked to diverse
biological and disease processes, yet the catalytic activity and substrate specificity of many
human protein lysine methyltransferases (PKMTs) are unknown. We screened over 40 …

Protein chips are widely used for high-throughput proteomic analysis, but to date, the low
sensitivity and narrow dynamic range have limited their capabilities in diagnostics and
proteomics. Here we present protein microarrays on a novel nanostructured, plasmonic gold …

Autoreactivity to histones is a pervasive feature of several human autoimmune disorders,
including systemic lupus erythematosus (SLE). Specific post-translational modifications
(PTMs) of histones within neutrophil extracellular traps (NETs) may potentially drive the …

Almost all humans with homozygous deficiency of C1q develop systemic lupus
erythematosus (SLE). The precise cellular mechanism (s) by which C1q prevents the
development of SLE remains unclear. In this study, we tested the role of C1q in the …

We developed a new, silicon-based peptide array for a broad range of biological
applications, including potential development as a real-time point-of-care platform. We used
photolithography on silicon wafers to synthesize microarrays (Intel arrays) that contained …

Obesity-related inflammation of metabolic tissues, including visceral adipose tissue (VAT)
and liver, are key factors in the development of insulin resistance (IR), though many of the
contributing mechanisms remain unclear. We show that nucleic-acid-targeting pathways …

Protein lysine methylation signaling is implicated in diverse biological and disease
processes. Yet the catalytic activity and substrate specificity are unknown for many human
protein lysine methyltransferases (PKMTs). We screened over forty candidate PKMTs and …

The mechanisms underlying development of ribonucleoprotein (RNP) autoantibodies are
unclear. The U1-70K protein is the predominant target of RNP autoantibodies, and the RNA
binding domain has been shown to be the immunodominant autoantigenic region of U1 …

Objective Type I interferon (IFN) signaling is a central pathogenic pathway in systemic lupus
erythematosus (SLE), and therapeutics targeting type I IFN signaling are in development.
Multiple proteins with overlapping functions play a role in IFN signaling, but the signaling …